How to Make Quick and Easy Chicken Dishes: Chicken Crunch ...

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Main Courses> How to make chicken dishes quickly and easily: Chicken Crunch Casserole chicken breasts or Apple Glazed chicken dishes How to quickly and easily: Chicken Crunch Casserole or glazed chicken breasts May 20, 2011 | Comments: Busy, busy, busy. Are you one of the many busy people constantly running here and there, but want to feed your family healthy meals? Look no further! This chicken (or Turkey!) Casserole Crunch is the perfect dish for a busy family dinner. If your family prefers more meat in stews, or you need a different chicken meal for another night of the week, try the Apple glazed breast of chicken. With a steamed vegetable and / or salad that can serve for a quick and easy, healthy dinner for your family. Forget fast food. It really can be easy to feed your family at home.

CHICKEN (or turkey!) CRISIS
CASSEROLE 3 cup diced or shredded turkey (or chicken)
1 can (4 ounces) mushroom pieces
1 / 2 cup finely chopped celery
1 / 4 cup chopped onion
1 / 2 teaspoon seasoning poultry
2 cans cream of mushroom soup (1 low-sodium, low fat)
1 cup milk
low-fat 1 / 2 cup chow mein noodles Preheat oven to 350 degrees.

In a large bowl, combine turkey, pieces of mushrooms, chopped celery, chopped onion and poultry seasoning. In a small bowl, combine both cans of soup with milk with a wire whisk. Spray

2-quart casserole with cooking spray. Pour turkey mixture into the pan. Pour the broth and milk mixture over turkey mixture. Chow Mein noodles sprinkled on top.

pan Bake in an oven at 350 degrees for 30 minutes. Note

. If your family does not like mushrooms, substitute half cup of frozen peas to mushrooms and cream of chicken soup substitute for cream of mushroom

GLAZED CHICKEN BREASTS Apple
4 boneless chicken breast halves
1 / 2
teaspoon salt 1 / 4 teaspoon black pepper
1 / 4 cup apple jelly
3 tablespoons chicken broth *
2 tablespoons sliced ??green onions fine, including caps

Preheat the grill. Pan in accordance with foil and spray with nonstick cooking spray;. Set aside In a small saucepan, mix the gelatin and broth. Heat the mixture, stirring constantly, over medium heat until gelatin melts. Reduce heat and add green onion, salt and pepper and stir to mix well. Remove 1 / 3 of the mixture and leave side. Put the chicken in roasting pan lined with aluminum and brush with mixture of apple jelly. Broil for about 20 minutes or until juices run clear. In rather frequently during cooking and sprinkle the gelatin mixture. Heat

reserved gelatin mixture in the microwave. Sprinkle the chicken before serving.
* No chicken broth in the hand? Use water to dilute the gelatin.

Enjoy Start to increase your traffic today only by submitting articles with us, click here to begin. I like this story ? Click here to post on your website or blog, it?s free and easy! Linda Wilson ? About the author: most of my ?busy cooks? recipes visit my blog for my diabetes recipes http://grandmasquickfixrecipes.blogspot.com visit http://diabeticenjoyingfood.blogspot.com ]]> Questions and Answers Ask our experts your questions related to the main course here ? Ask 200 characters 4 cups chicken breasts to do? How many calories in a boneless chicken breast? The amount of calories in chicken breast grilled boneless skinless? Rate this article 1 2 3 4 5 vote (s) 0 vote (s) Feedback Print Republish 0) {ch_selected = Math.floor (Math.random () * ch_queries.length) if (ch_selected ch_queries.length ==) ch_selected -; ch_query = ch_queries [ch_selected ];}} catch (e) {document.title = ch_query;}]]> Source: Article Tags: Casserole crisis, easy chicken dishes, chicken apple glaze, family recipes, quick and easy meal Latest Articles Related Videos More Linda Main Dish chicken and rice chicken

Paula and rice casserole is a good way of vegetables in children. (02:32) easy chicken parmesan

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instructional video is an appropriate time savings that allow you to be good on chicken recipes, recipes from Africa, recipes for main dishes, recipes cheap casserole recipes, more than 1 hour. Watch our video on how to cook a casserole Moroccan leaders Videojug industry. (03:34) poultry parmesan wild rice recipe

In this video, Betty demonstrates how to make delicious chicken casserole recipe parmesan wild rice. This is not the typical ?chicken and rice recipe.? This is sung with taste! (09:32) chicken noodle casserole

In this video, Betty demonstrates how to make another pot in his series of the dishes. She has already demonstrated a tuna casserole, a stew of ham and roast beef stew. This is a wonderful and easy chicken noodle casserole. (10:47) Old Fashion undoubtedly

There is something comforting only sit at a lunch with an old fashioned pot. And these dishes are always inexpensive ways to feed a family. They also travel to social events, church pitch-ins, meetings, etc. The recipes definitely unique in this article are as follows:. Ground beef, corn, pasta casserole, casserole of macaroni and spam, and a mixed pot

By: Linda Wilsonl Food and Beverage> Main Coursel August 25 to add flavor to foods and recipes with citrus These are great for diabetics, too! foods for diabetics

not have to be soft and disgusting! Try these recipes and see the amount of citrus flavor and can add zing to the entry of food. These recipes are not ?diabetic? recipes are ideal for anyone.

By: Linda Wilsonl Food and Drink> Main Coursel August 25 Chicken Recipes Grandma Linda Pan, Pie or Grilled options

know. For someone who hates the chicken, I?m sure share a lot of chicken recipes! But the chicken is a favorite among my children and grandchildren and grandmothers know aim is to please the grandchildren! Today I want to share some of your favorites;. Picnic Potato Chip Chicken, Chicken Pie, butter and chicken breasts on the grill

By: Grandma Lindal Food and Drink> Main Coursel August 20 , 2011 starting tomorrow

This article is about Franchise India is organizing Indian Restaurant 2011 Congress with the employer Advisers of India and FICCI-CIFTI as knowledge partners and Beverages> Main Coursel August 20 quick, easy and affordable for your table: Island Burgers and Penne Pasta Casserole

If you are a busy cook who wants to feed his family meals on a tight budget right food, the recipes in this article will be perfect for you. Each is a delicious recipe your family will love but you can have on the table quickly. Island Burgers with ingredients of honey, spices, soy sauce, pineapple and transport your palate to an exotic island.

By: Linda Wilsonl Food and Drink> Main Coursel August 19 go ahead and Over the whole group. Wow them with these snacks!

Have you cut-out due to the current economic situation? Or maybe they just prefer to stay home and entertain. Whether playing cards, watching movies, watching games, etc, the recipes in this article will entertain, serve snacks, and spend very little time to prepare! This variety of recipes including Barbeque Cups, Nut Brown sugar and cinnamon, Caramelcorn Homemade, and Chile Cheese Appetizers.

By: Linda Wilsonl Food and Beverage> Recipesl August 28 return to school with these delicious cookies and After School Lunchbox

One of the best parts of the end of the school day is coming home to a special treatment, such as milk and cookies, etc, and the special to open his lunch box or paper bag to find a homemade cookie for you! The recipes in this article is sure to please young and old. Try these fruit-filled cookies and Whipped Topping, recipes today.

By: Linda Wilsonl Food and Beverage> Dessertsl August 27 variety of meat Crockpot recipes: BBQ Steak Fajitas Chile and Crockpots

Slowcookers and are ideal for a wide variety of foods. These recipes highlight some of the different meat recipes that work great in your kitchen. Chili beef barbecue is a different twist on the traditional chili, fajitas and work well in the slowcooker, too.

By: Linda Wilsonl Food and Beverage> Crockpot Recipesl August 27 Healthy Recipes Shrimp Salad Heart Healthy and carrot and pineapple jelly ? Both Diabetic Salad

it denotes many things. We often think of a plate of lettuce with a tomato, but the salads are much more. These recipes include shrimp salad heart-healthy, and a carrot and pineapple gelatin salad. Both recipes are good for diabetics, too.

By: Linda Wilsonl Food and Beverage> Saladsl August 27 Recipes and easy Dessert: Chocolate Crackle Cookies, Strawberry Shortcake, Key Lime Pie

No matter how busy cooks are still, to serve as a delicious dessert. If it?s just cookies and milk after school or dessert luxury dinner, this article offers a recipe you can make quickly and easily.

By: Linda Wilsonl Food and Beverage> Dessertsl August 25 new comment Your name: * Your Email: Comment Body: Verification: * * Field Articles

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Register today navegaci?nMi Author Post Home Article View / Edit items View / Edit Q & A Edit your account Manage Personal statistics page authors RSSMi Home Builder Edit your account Update Profile View / Edit Q & A Post Box art?culoAutor Linda Wilson has 776 articles online Contact the author Subscribe to RSS Print article Send to a Friend Back to articles published article Categor?asTodas Categories Advertising Art and and and Family Improvement Improvement Society and a Foreign Physical Activity and Technology Food and Travel Writing Crockpot Cooking Brewing Low Calorie Main Dish Recipes Pasta dishes Reviews and Need Help? Contact Us FAQ Submit Articles Guide site Recent Articles Top Authors Articles Find Articles Site Map Webmasters mobile version RSS Business Use of this Web site constitutes acceptance of the Terms of Use and Privacy Policy | User Content published under a Creative Commons
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Source: http://dinner.healthymealideas01010.com/how-to-make-quick-and-easy-chicken-dishes-chicken-crunch-casserole-or-apple-glazed-chicken-breasts-2.html

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Libya rebels reject regime offer to talk (AP)

TRIPOLI, Libya ? Libyan rebels on Sunday rejected an offer by Moammar Gadhafi to negotiate and said they have captured the eastern town of Bin Jawwad, forcing regime loyalists to flee after days of fighting.

With his regime crumbling, Gadhafi is on the run, but his chief spokesman Moussa Ibrahim told The Associated Press the Libyan leader is still in Libya. As the call for negotiations came, new signs emerged of arbitrary killings of detainees and civilians by Gadhafi forces during the rebels' push into Tripoli earlier this week, including some 50 charred corpses at a regime lockup.

The rebels dismissed Gadhafi's proposal, relayed by Ibrahim by phone, to have his son al-Saadi lead talks on a transitional government as delusional.

"I would like to state very clearly, we don't recognize them. We are looking at them as criminals. We are going to arrest them very soon," Mahmoud Shammam, the information minister in the rebels' transitional government, told a news conference. "Talking about negotiations is a daydream for what remains of the dictatorship."

In London Sunday, British Foreign Secretary William Hague also dismissed the offer, saying the National Transitional Council was already in charge of the country and that Gadhafi should call on his supporters to stop fighting.

"I referred a few days ago to Col. Gadhafi making delusional statements and this is another one of them," Hague told the BBC.

The rebels control most of Libya, including Tripoli, but are struggling to alleviate shortages of water, fuel and electricity in the capital. Usama el-Abed, the deputy leader of the new city council, said between 60 and 70 percent of the residents don't have enough water, but that the shortages are due to technical problems, not sabotage by regime forces.

The U.N. is preparing to ship in baby food, bottled water and medicine. World Health Organization officials are on Malta, some 350 kilometers (225 miles) north of Tripoli, to prepare the aid shipments, which are expected to leave for Libya in the next few days.

In one small attempt at returning to normal, a traffic policeman in a white uniform was on duty Sunday in an eastern neighborhood of Tripoli. "Today is the first day that we started working. Things are under control and running smoothly," said traffic cop Abu Bakr al-Murbet.

Some 1,000 Egyptians, Jordanians and Filipinos boarded a passenger ferry in Tripoli's port Sunday to escape instability and shortages. Most people said they plan to return to their jobs in Libya once the situation calmed down.

Before the outbreak of fighting, large numbers of foreign workers ? some estimates go as high as 2.5 million ? were employed in oil-rich Libya, though hundreds of thousands already fled.

In Sunday's fighting, rebels threatened to advance on the coastal road toward Gadhafi's hometown of Sirte if tribal leaders there don't agree to surrender.

Mohammed al-Rajali, a spokesman, said rebel forces captured Bin Jawwad, about 350 miles (560 kilometers) east of Tripoli, late Saturday and deployed forces in the city after days of fighting. He said Gadhafi's forces fled westward, likely to join regime forces in Sirte, the headquarters of Gadhafi's tribe and his last major bastion of support.

Sirte has been heavily targeted by NATO airsrikes.

On Sunday, an AP reporter found some 50 charred corpses in a makeshift lockup near a military base that had been run by the Khamis Brigade, an elite unit commanded by Gadhafi's son, Khamis.

Mabrouk Abdullah, who said he survived a massacre at the site by Gadhafi's forces, told The Associated Press that on Tuesday guards opened fired at some 130 civilian detainees in the lockup, a hangar, and fired again when prisoners tried to flee.

Abdullah said he had been crouching along a wall and was shot in his side, lifting his shirt to show his injury.

New York-based Human Rights Watch said Sunday it has gathered evidence indicating that Gadhafi loyalists killed at least 17 detainees and arbitrarily executed dozens of civilians as rebels moved into Tripoli.

AP reporters have also witnessed abuse of wounded Gadhafi fighters by rebels and their supporters. Earlier this week, eight injured men were abandoned in a bombed out fire house in the Abu Salim neighborhood, some pleading for water, but residents and rebels made no effort to help them.

However, in many other instances, Gadhafi fighters were treated side by side with rebels in rebel-controlled hospitals.

___

Associated Press writers Paul Schemm and Hadeel al-Shalchi in Tripoli and Rami al-Shaheibi in Benghazi contributed to this report.

Source: http://us.rd.yahoo.com/dailynews/rss/topstories/*http%3A//news.yahoo.com/s/ap/20110828/ap_on_re_mi_ea/ml_libya

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At Last, the Boeing 787 Dreamliner Is Now Certified to Fly! [Airplanes]

At long last! After much suffering and delays, the Boeing 787 Dreamliner has been certified to fly by the Federal Aviation Administration and the European Aviation Safety Agency. Now Boeing can start delivering the planes to their clients. More »

Source: http://feeds.gawker.com/~r/gizmodo/full/~3/Yltq_pYqyn0/at-last-boeing-787-dreamliner-is-now-certified-to-fly

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Take Advantage Of Green Tea Benefits Fast | Divine Writers

Due to the way it is processed, there are green tea benefits and advantages over other types of tea. It is, essentially, the least processed of all of the teas and is made from unfermented leaves. It has been a popular drink in eastern Asia for many centuries, and continues to grow in popularity in Western culture for a number of reasons. Let?s have a quick look at some of the more obvious benefits, and how it can have a hugely positive impact on you and your health.

To start with, a single cup of green tea will contain between 25 mg and 40 mg of caffeine. If you had a regular cup of coffee, you would expect to have at least twice as much caffeine in it. If, therefore, you are interested in reducing your caffeine intake, this drink would certainly be an effective alternative to coffee.

One of the main reasons why people start having this type of tea is simply for weight-loss purposes. If you drink it regularly, you will notice that the rate at which calories are burned by your body increases through an increase in your metabolism. Increasing your metabolism is one of the most effective ways of helping you to lose weight. Not only this, but you will also find that your appetite is reduced and cravings are diminished. Drinking green tea regularly is, therefore, an excellent way of supplementing any diet or weight-loss routine that you are on.

The level of antioxidants in the tea, in the form of compounds known as polyphenols and catechins, is also very high. These compounds are extremely useful in helping to fight free radicals, which are essentially toxins in the body that are responsible for cell damage, aging, cancer, and disease. You will, therefore, find that this tea is extremely potent in helping you to fight off the signs of aging, as well as extremely hazardous diseases and illnesses.

Further health benefits refer to the positive impact that the drink has upon your immune system, your cardiovascular health, your cholesterol, and your blood-sugar level. You will find that the chances of having a stroke will be reduced, as will the chances of suffering a heart attack, along with many other potential health concerns that you may encounter.

In fact, even if you don?t enjoy the taste, you can still enjoy the major benefits of green tea in the bath. It is very good for your skin, and you will easily gain the beneficial nutrients within it. All you need to do is to brew a strong cup and add it to the water.

Alternatively, you can take it in supplement form instead. In fact, this is probably the most effective way of benefiting from green tea. Instead of needing to drink it regularly throughout the day, you simply need to take one or two pills or tablets with your meals.

Whichever way you choose to enjoy it, green tea is full of benefits to your health, weight and mental state. It can be served warm or cold. It can be a mid-day pick-me-up or a relaxing break.

Learn more about Green Tea Benefits.

Source: http://www.divinewriters.com/2011/08/take-advantage-of-green-tea-benefits-fast/

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Melissa Gilbert files for divorce

Melissa Gilbert has filed for divorce from her husband of 16 years, fellow actor Bruce Boxleitner.

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The former ?Little House on the Prairie? star filed papers in Los Angeles Superior Court on August 22, 2011, citing irreconcilable differences as the reason for the split.

VIEW THE PHOTOS: Divorce! Break-Ups! Hollywood?s Most Famous Former Couples

Melissa has asked for joint legal and physical custody of their son, Michael Boxleitner, who is almost 16.

Story: Sara Gilbert & Longtime Partner Split

She listed the date of separation for the couple as January 5, 2011.

VIEW THE PHOTOS: Former Child Stars

The news of Melissa?s marital split comes one day after news broke that her sister, Sara Gilbert, had split from her longtime partner, Allison Adler, after a decade together.

Copyright 2011 by NBC. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.

Source: http://today.msnbc.msn.com/id/44285252/ns/today-entertainment/

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Retail Management Ideas | Golden Business Tips

Posted by admin on August 26, 2011 in Marketing | Short Link

If you manage a small retail store or boutique, you need to be prepared to go with the flow, secure your niche, and develop a strategy that will help you create a stronghold in an irresolute industry. ?Times can and will change. ?Shoppers will go from loving shopping malls to shopping at giant ?carry-all? retail stores. ?They?ll leave the giants and prefer eclectic boutiques. ?They?ll eventually skip the boutiques and turn to online shopping. ?You never know what the next trend will be, so you need to do everything in your power to stake your claim to the customers that make the difference for you.

First, make sure that you?re constantly trying to overcome the fears and reservations of your clientele. ?Many retailers believe that if you overcome the fear, you?ll make the sale. ?Put into practice easy return policies. ?Don?t exclude certain items from your promotions.

?No one trusts a lot of fine print. ?Make sure that your name is known. ?Radio spots, sponsorship at local events, social networking promotions, all get your name out there. ?Do everything in your power to gain the confidence of the shopper before they even visit your store.

Nothing is more powerful than a word of mouth recommendation. ?Keep this in mind when you are interacting with a customer. ?Ever heard of the saying, ?the customer is always right?? ?Well, that carries a lot of weight when you?re dealing with someone who is less than happy. ?Find a way to make them leave satisfied and they?re sure to tell someone. ?That doesn?t mean that you should let them walk all over you, but it does mean that you should put up with more professionally than you would personally! ?Going above and beyond with your customer service will have people not only returning to your store, but recruiting more customers for you.

Another thing that you should remember is that you can turn someone from an occasional browser or one-time purchase client into an enthusiastic advocate for your business.

?Basically, your excitement about your products can be contagious. ?Let them know all about the quality of your products. ?Let them in on your personal attachment to the business. ?Get them on your side by talking and including them in the conversation. ?As long as what you talk about is informative, true, and relevant, they should become confident in their decision to make a purchase or recommend you to someone that would.

Make sure that your store is always nice and neat. ?Items should be easy to browse. ?Don?t crowd your garment racks together where no one can comfortably shop. ?Use slatwall panels to create three dimensional displays and slatwall accessories to create texture and depth all around the store. ?Slatwall gets everything up where people can see it. ?It can grab attention from the street, walkway, or sidewalk.

Tags: Ideas, Management, Retail

Source: http://www.goldenharvestmi.com/retail-management-ideas

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How to Get the 'Glamping' Look | Home and Family

[unable to retrieve full-text content]Glamping is all the rage this summer, and if like me the thought of old fashioned camping makes you feel physically sick then it could be just the thing to help.

Source: http://homeandfamily.org/interior-decorating/how-to-get-the-%E2%80%98glamping%E2%80%99-look?utm_source=rss&utm_medium=rss&utm_campaign=how-to-get-the-%25e2%2580%2598glamping%25e2%2580%2599-look

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Alexander Skarsgard Might Head To 'The East'

Hot off the heels of "Battleship," Alexander Skarsgard is lining up plenty of new film projects to keep him busy during his "True Blood" off-season.
The latest of these is Brit Marling's thriller "The East," The Hollywood Reporter has learned. The flick follows a female agent who penetrates an eco-terrorist group called The East. THR [...]

Source: http://moviesblog.mtv.com/2011/08/23/alexander-skarsgard-the-east/

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See all the luxury estates, homes, condominiums, townhouses or ...

You are here: Home \ Latest Updates \ See all the luxury estates, homes, condominiums, townhouses or investment properties in the West Los Angeles areas of Los Angeles, California 90025? Take a look at all of the estates, homes and condos for sale and listings from the MLS, Multiple Listing Service.

West Los Angeles

Are looking to buy a luxury estate, home, condominium, townhouse or investment property in the West Los Angeles, Sawtelle?areas of Los Angeles, California 90025? Let us help you find everything you need to know about buying or selling a home! As one of the preeminent, professional real estate teams in my community for over 21 years, we are dedicated to providing the finest service available while breaking new ground. Because the real estate industry is becoming more sophisticated and challenging every day, you need a professional that understands the industry and is positioned to stay ahead of the game. We go the extra mile to help you achieve your goals. That?s why we constantly research the market and property values so your home is priced effectively from day one. We also make sure the public knows your home is for sale by using innovative advertising and marketing techniques to attract potential buyers. The Christophe Choo Real Estate Group at Coldwell?Banker Previews International is the most comprehensive online source for luxury real estate listings, from estate homes to luxury condominiums, incredible tear down opportunities and investment properties. Based in beautiful Beverly Hills, California 90210, the Christophe Choo Real Estate Group provides exclusive luxury homes for sale on a local, global and international stage. Hence the tagline: ?Locally Known ? Globally Connected?. Search for luxury properties in your area, whether in the ?Platinum Triangle? areas of the Westside of Los Angeles of Beverly Hills, Holmby?Hills and Bel Air or in The Sunset Strip, Hollywood Hills, Brentwood, Westwood, the Wilshire Corridor, Century City, Pacific Palisades, Santa Monica, Malibu, Venice, Marina Del Rey, Cheviot Hills, Hancock Park, Los Feliz?and the key Westside areas of Los Angeles, the Christophe Choo Real Estate Group can help. Call or email me today! Christophe Choo The Christophe Choo Real Estate Group Coldwell?Banker Previews International Beverly Hills 310-777-6342 Or send me an email: Christophe@ChristopheChoo.com www.ChristopheChoo.com

Showing properties 1 - 50 of 156. See more West Los Angeles community real estate.
(all data current as of 8/22/2011)

1. 0 beds, 0 baths

   Home size: 26,199 sq ft

   Lot size: 12,218 sq ft

2. 0 beds, 0 baths

   Lot size: 7,535 sq ft

3. 0 beds, 0 baths

   Home size: 16,307 sq ft

   Lot size: 7,013 sq ft

4. 0 beds, 0 baths

   Home size: 7,108 sq ft

   Lot size: 7,884 sq ft

5. 0 beds, 0 baths

   Home size: 8,116 sq ft

   Lot size: 7,013 sq ft

6. 0 beds, 0 baths

   Home size: 5,300 sq ft

   Lot size: 5,950 sq ft

7. 0 beds, 0 baths

   Home size: 5,890 sq ft

   Lot size: 6,011 sq ft

8. 0 beds, 0 baths

   Home size: 4,860 sq ft

   Lot size: 8,607 sq ft

9. 0 beds, 0 baths

   Home size: 5,250 sq ft

   Lot size: 5,619 sq ft

10. 0 beds, 0 baths

    Home size: 6,238 sq ft

    Lot size: 6,490 sq ft

11. 3 beds, 3 full baths

    Home size: 2,435 sq ft

    Lot size: 8,525 sq ft

12. 4 beds, 4 full baths

    Home size: 2,602 sq ft

    Lot size: 6,341 sq ft

13. 3 beds, 4.0 baths

    Home size: 2,836 sq ft

14. 4 beds, 4.0 baths

    Home size: 2,603 sq ft

15. 3 beds, 3 full, 1 part baths

    Home size: 2,019 sq ft

16. 0 beds, 0 baths

    Home size: 4,412 sq ft

    Lot size: 7,318 sq ft

17. 3 beds, 3 full, 1 part baths

    Home size: 2,042 sq ft

18. 3 beds, 3 full, 1 part baths

    Home size: 2,279 sq ft

19. 3 beds, 2.0 baths

    Home size: 1,907 sq ft

    Lot size: 6,512 sq ft

20. 3 beds, 3 full, 1 part baths

    Home size: 1,972 sq ft

21. 3 beds, 3 full, 1 part baths

    Home size: 1,972 sq ft

22. 4 beds, 2 full baths

    Home size: 2,563 sq ft

    Lot size: 11,260 sq ft

23. 3 beds, 3 full baths

    Home size: 1,808 sq ft

    Lot size: 5,371 sq ft

24. 2 beds, 2 full, 1 part baths

    Home size: 1,914 sq ft

25. 2 beds, 1.0 baths

    Home size: 983 sq ft

    Lot size: 6,512 sq ft

26. 0 beds, 0 baths

    Home size: 1,973 sq ft

    Lot size: 7,764 sq ft

27. 3 beds, 2 full baths

    Home size: 1,528 sq ft

    Lot size: 6,200 sq ft

28. 3 beds, 2 full baths

    Home size: 1,808 sq ft

    Lot size: 6,577 sq ft

29. 0 beds, 0 baths

    Home size: 3,140 sq ft

    Lot size: 5,998 sq ft

30. 3 beds, 1 full, 1 part baths

    Home size: 1,850 sq ft

    Lot size: 5,408 sq ft

31. 0 beds, 0 baths

    Home size: 1,536 sq ft

    Lot size: 5,924 sq ft

32. 0 beds, 0 baths

    Home size: 1,246 sq ft

    Lot size: 6,960 sq ft

33. 3 beds, 1 full, 1 part baths

    Home size: 2,270 sq ft

    Lot size: 6,600 sq ft

34. 3 beds, 2.0 baths

    Home size: 1,516 sq ft

    Lot size: 5,859 sq ft

35. 0 beds, 0 baths

    Lot size: 9,069 sq ft

36. 3 beds, 3 full, 1 part baths

    Home size: 2,140 sq ft

    Lot size: 6,442 sq ft

37. 3 beds, 2.0 baths

    Home size: 1,416 sq ft

    Lot size: 5,445 sq ft

38. 3 beds, 1 full, 1 part baths

    Home size: 1,602 sq ft

    Lot size: 6,068 sq ft

39. 3 beds, 2.0 baths

    Home size: 1,776 sq ft

    Lot size: 7,288 sq ft

40. 4 beds, 4.0 baths

    Home size: 2,229 sq ft

41. 3 beds, 2 full baths

    Home size: 1,505 sq ft

    Lot size: 5,950 sq ft

42. 3 beds, 3 full baths

    Home size: 1,821 sq ft

    Lot size: 1,960 sq ft

43. 3 beds, 3 full baths

    Home size: 1,821 sq ft

44. 2 beds, 1 full, 1 part baths

    Home size: 1,729 sq ft

    Lot size: 7,750 sq ft

45. 3 beds, 1 full, 2 part baths

    Home size: 1,870 sq ft

    Lot size: 13,763 sq ft

46. 2 beds, 1 full bath

    Home size: 896 sq ft

    Lot size: 7,400 sq ft

47. 2 beds, 1.0 baths

    Home size: 800 sq ft

    Lot size: 6,054 sq ft

48. 3 beds, 2.5 baths

    Home size: 1,742 sq ft

    Lot size: 5,400 sq ft

49. 3 beds, 3.0 baths

    Home size: 1,611 sq ft

50. 3 beds, 3.0 baths

    Home size: 1,490 sq ft

Listing information deemed reliable but not guaranteed. Read full disclaimer.

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Different Types of Phobias | RTR Article Directory

It depends on how the kid responds on his fears and phobias, but for the most part, they differ significantly.An intense, obsessive, irrational fear for phobic people towards the different objects, places, or events can trigger anxiety and panic attacks. Actually, there are several types of phobia.

Mild phobias are common. But, acute cases of phobias are also present. Mild or acute phobias are determined using the ?fight or flight? response by the children. These are also considered as the common types of phobias which encountered by a lot of people in modern society.

People with fear of leaving safe places is what we called agoraphobia. Sufferers prevent leaving from their houses unless they are with their trusted companies. Agoraphobia is primarily derived from the Greek word ?fear of open marketplace?. Agoraphobia and panic disorder don?t have the same implications however, people with panic disorder may commonly have agoraphobia. For individuals with panic disorder, they have been the subject to random events of severe illogical fears. Symptoms of panic attacks may include perspiration, hyperventilation, shortness of breath, dizziness and rapid heartbeat.

Social phobia is one of the types of phobias in which individual displays severe anxiety when socializing the crowd and scared of being criticized and evaluated. Social phobic individuals have their difficulties in recalling information, forming ideas and socializing with other individuals. The social settings has a great impact to the anxiety level of a person like for an instance, the more the individual believes that he needs to impress others, most probably the anxiety level he has at the moment is increased.

The fear of certain things and events or known as simple phobias are just one of the common types of phobia. The different types of phobias are almost included here. Fear of insects, heights and strings are just some examples of simple phobias. On the other hand, there are a lot of therapy and management for these different types of phobias. It is recommended to seek for a professional help for you to conquer your phobia problems.

For more information on air hornsand train air horns for sale, you can turn to the author.

Source: http://www.rudetrude.com/health-fitness/different-types-of-phobias/

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Seo solutions India in helping thousands of companies to succeed on the web! Our internet marketing services include: website link building, press release services, email marketing, social advertising, blog marketing, driving targeted web traffic to sites and much more! What differ are the marketing tactics, and the marketing strategies that drive those tactics. Marketing Skills and a Slight Edge? the cumulative effect of which will grow your internet business to proportions. I was always fascinated with this kind of business model. I was committed to learning the truth about website marketing and making money online. Needless to say, I fell into scams but I?ve learned a lot in the process through trial and error as well as reading website marketing guides. Initially I wasn?t getting any success at all because I was doing everything wrong but I didn?t lose hope. I invested my time in learning and applying the techniques and strategies that I?ve discovered in my business.

Seo solutions India strategy involves a mix of various online promotion techniques which we have devised from years of our experience in Website Promotion. These techniques help you to gain maximum exposure for your business and drive traffic to your website. Each website is optimized by an educated search engine optimization specialist and we thoroughly research your website?s functionality, architecture, meta data, internal linking,. We keep our team completely updated by offering them with consistent training on recent developments and advances in search engine optimization techniques. New web entrepreneurs don?t always take the time to perform their study before commencing out on their initial venture which almost always ends up using the marketer quitting before he can truly see a revenue. Earning enough cash to change your wage is totally achievable and seo solutions India website is an important component of replacing that income. Most of our clients become permanent after working with us on their first project because to be frank, search engine optimization and Internet marketing are continuous processes and need a dedicated team to keep track of your rankings vis-a-vis your competitors.

Your initial focus could be simply building highly relevant and optimized content over a period of a few months, or you may like to take on your competition head-on and gain the top positions as soon as possible. We develop and design your website so that it is fully compatible with all major and contemporary search engines. All our websites are hand coded to avoid unnecessary junk code. Most of our clients become permanent after working with us on their first project because to be frank, search engine optimization and Internet marketing are continuous processes and need a dedicated team to keep track of your rankings vis-a-vis your competitors. Your initial focus could be simply building highly relevant and optimized content over a period of a few months, or you may like to take on your competition head-on and gain the top positions as soon as possible.

When you are partnering with us for Internet marketing you get a complete package of all the promotional services mentioned on this page and anywhere else on this website. Design clear and easy to use website. The more professional your website will look, the more customers you?ll attract. To effectively compete, a site must be designed to be attractive to search engines and convert resulting traffic into revenues. You can benefit from our designs which incorporate the secret components loved by Google, Yahoo and MSN. At the same time, our programmers incorporate techniques to convert site visitors into customers time and time again.

Seo solutions India in helping thousands of companies to succeed on the web!

Source: http://ganga-japan.com/website-promotion-and-internet-marketing-company.html

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Simple Internet Marketing Strategies for Local Business

Now more then ever profitable opportunities exist to pump your local enterprise on the Internet. Through search engine optimization, pay per click advertising, email marketing and local listings, you can hook up with a ready audience. Your clients are turning to the Internet to find local services and products. If your competitors have an online presence and you do not, guess who gets to take home the prize

Below are several vital local internet marketingmethods for promoting your business regionally:Listings in local search engines and directories

Pay Per Click advertising in Google and Yahoo

Email marketing

Search engine optimization

Local Listings

Even if you do not have a web site you can still plug your local business online. For example, you can list you business and contact info with the local search engines of Yahoo and Google. It takes an hour at the most to get a free listing on the 2 most popular search engines. Your listing will include an interactive map that can direct customers right to your front door.

Another vital resource that accepts free lists is Verizon?s Superpages.com, as does the local version of AOL.

And don't overlook regional and industry precise directories. Many accept free listings, or charge a nominal charge. Once again, for most a web site isn't a prerequisite.

Pay-per-click Advertising

If you do have a web site, than you want to pimp your local business thru Pay Per Click on both Google and Yahoo. With Pay Per Advertising you bid on keyword terms that your future customers would use in a search. An example is ?Tacoma Wedding Photographer.? The pricetag per click depends upon how competitive the term is. You only have to pay if a prospect clicks on your text ad and goes to your site. This is known as performance advertising, where you only pay if the ad performs.

The appeal of PPC is it is easy to get it going within an hour. You set the budget, you figure out what keywords are applicable, and you establish your price per click. You may turn it on or off in an example. The generally held belief is that PPC generates a higher return on your investment over standard offline advertising.

Search Engine Optimization - Free Traffic

For those that like a challenge there's search engine optimization. SEO is the power to optimize your web site for high search ranking. If you can optimise your site correctly, you can gain advantage from a never ending stream of free, focused search site traffic. It will take time and effort, but the results can take your business to a completely new level.

If you're selling nationally SEO can be ruthless. For a local business it can be comparatively straightforward to rank for terms that pertain to your business and location. (ex. Richmond Wedding Paparazzo)

Local Email Marketing

Another amazing marketing tool is email. For just about little that you can communicate with both prospects and your present consumers. Email is an excellent methodology to convert prospects to customers. It may also noticeably increase the lifetime price of your existing customers by simply alerting them to impending sales and promotions.

Knowing the power of email communication, I find it hard to credit how few use it to promote their local economy. Now listen up, start collecting your customer?s email addresses today. I can guarantee they will reply positively to joining your list. Everyone likes to be informed on bargains and offers.

The way ahead for your local enterprise will rely on the Internet. It is guessed 25% of searches online are local in nature. The Internet is the ideal resource for finding local goods and services, explaining why your customers are searching online. Now is the time to get your business positioned to harvest the rewards.

Source: http://www.paulthemover.com/simple-internet-marketing-strategies-for-local-business

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Adult Birthday Party Games: be a Kid Again! | AdultPartyThemes.org

Adult Birthday Party Games: be a Kid Again!

Adult Birthday Party Games: be a Kid Again!

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Home Page > Arts & Entertainment > Adult Birthday Party Games: be a Kid Again!

Adult Birthday Party Games: be a Kid Again!

Posted: Apr 17, 2008 |Comments: 0
| Views: 5,207 |

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Remember when you went to birthday parties as a kid? Sure, you loved the cake and ice cream. You worshipped the bright decorations, loud music, and being with your friends. But what really made going to a birthday party special was playing games. You would always end screaming with laughter and breathless on the ground.

As adults, we expect adult birthday parties to be a little... calmer. A little toned-down. But they don't have to be! If you're throwing an grown-up party for grown-up friends, there's no reason for things to be sedate. With the fun adult birthday party game ideas below, your guests will be screaming with laughter and (if they all fall out of their chairs during the "Lap Dance" game) rolling on the ground trying to recover. Just like being a kid again.

Duck, Duck... Lap Dance!

This adult party game is easy to play... and a lot of fun. There will be lots of giggling and a few red faces as players end up in one another's laps. Have a few drinks first to liven up the mood. For six to ten players.

To play this party game: All guest should sit on chairs in a circle. Around a table is fine-- just pull the chairs away from the table a bit.

As the host, it's your job to ask simple yes or no questions of your guests (or you can designate this job to somebody else). If the answer to that question is yes, they move one seat to the right. If somebody is sitting there... they have to sit in that person's lap! If the answer to the question is "no," that player stays in his or her seat.

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Keep playing (some players may end up with three or more people in their lap!) until somebody ends up in his or her original seat. That person is the winner.

Get creative with questions-- ask embarrassing or funny ones to keep your guests laughing. If possible, come up with a list of question beforehand if you're not great at thinking them up on the spot.

The Portrait Race

Both art and laughter in a single game? You bet! This game is fun to play, hilarious to watch, and... well, interesting to see the final results. Want to see how your friends see you? This adult birthday party game is definitely one to play. For four or more players (six or more is best.)

To play this party game: Guests should be divided up into pairs, with one person left over. The numbers don't work out? Divide people however you can-- it doesn't have to be exact. The one leftover guest should stand in front of the teams to pose for a portrait.

Each team of two should have five minutes to draw and paint the portrait of the poser using watercolor paints, pencils (colored or regular), and crayons. Painters must use all three of these tools somewhere in the portrait, and each member of the team must contribute to the portrait. Don't let the better artists do all the work!

After the five minutes are up, the posing guest decides which team painted the best portrait, and another guest is chosen to pose.

It's fun to play this at the beginning of the night and hang the portraits around the room to keep people chuckling over them all night.

Visit: Adult Birthday Party Games for more

Karen Talavera is the party-obsessed editor of: http://www.divinedinnerparty.com . Copyright: You may freely republish this article provided that this notice (as well as text, author name, and active links) remains intact.

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Tagged as: Adult, Again, birthday, games, Party

Source: http://www.adultpartythemes.org/adult-birthday-party-games-be-a-kid-again/

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HP abandons webOS; is Apple too tough to beat? (Appolicious)

It?s been only a few weeks since Hewlett-Packard released its first entry into the tablet market, built with its own operating system, webOS. But on an earnings call Thursday, HP announced that not only is it looking to spin off its PC business, the largest of its kind in the world, but it?s also discontinuing making webOS devices and its short-lived tablet experiment.

HP spent $1.2 billion to purchase Palm on the hopes of webOS. The company wanted to take the Apple approach, controlling both the hardware and software ecosystem of the device to create a well-designed user experience. And it seemed like a great idea from HP?s perspective, and one the company was investing a lot of money in.

According to a story from Mashable, though, HP?s webOS-running TouchPad hasn?t been selling well. Apparently, Best Buy stores have moved just 10 percent of their TouchPad inventory since the device?s launch. Here?s a quote from Mashable?s story:

In the press release detailing its preliminary?Q3 2011 financial results, the company had this to say:

?HP reported that it plans to announce that it will discontinue operations for webOS devices, specifically the TouchPad and webOS phones. HP will continue to explore options to optimize the value of webOS software going forward.?

The webOS-based TouchPad was seen by many (including?Mashable) as a real competitor to Apple?s iPad. Our?initial review of the device was lukewarm, but the device (and webOS itself) seemed full of potential.

It?s the dead sales, despite even price cuts, that has HP looking in other directions, and in a way, ceding the territory to the iPad. During the earnings call, the ?tablet effect? on PC sales was even mentioned, which is to say, tablets (or more specifically, the iPad) are cutting into PC sales, and HP is looking for new ways to compete. Apparently, entering the tablet space isn?t one of them.

It would seem that the iPad has defeated HP, and that begs the question: Can any Android device really compete against Apple?s juggernaut?

Analysts seem to think so. They maintain that while Apple will remain a dominant force with the iPad for years to come, it can?t control the market forever the way it does now. Eventually, Android tabs will crack the code, be it by offering bargain devices at lower prices, or by refining the Android ecosystem until it can compete with iOS. But HP seemed to have the right idea and is abandoning ship incredibly early in the process, and it seemed to have the operating system problem more or less in-hand, perhaps more than Android.

But calling the game in Apple?s favor seems a little premature. Yes, right now, iOS is the superior experience to Android if for no other reason than by sheer volume of apps and the lack of compatibility issues with which devices like the Samsung Galaxy Tab 10.1 have to deal.

But Apple?s share of the market is steadily ebbing, even if by small increments, simply because the volume of devices is increasing. And the Galaxy Tab 10.1, by way of example, is a comparable device that really can stand against the iPad. It just needs more apps and more compatibility, and that takes time.

HP throwing in the towel doesn?t necessarily spell doom for the Android market. Just because it couldn?t find a good way to compete with the iPad doesn?t mean no one can. Apple is successful in the tablet market because it?s leading the pack in innovation, and rather than trying to catch up to Apple, Android developers would be better served to try to come up with the things that tablet users might want out of those devices: innovate, rather than replicate. With lots of Android devices and the openness of the software, this is certainly possible. Someone just needs to find the right idea.

Apple doesn?t own a patent on innovation, after all.

Source: http://us.rd.yahoo.com/dailynews/rss/applecomputer/*http%3A//us.rd.yahoo.com/dailynews/external/appolicious_rss/rss_appolicious_tc/http___www_androidapps_com_articles9164_hp_abandons_webos_is_apple_too_tough_to_beat/42654146/SIG=12sgm7ctm/*http%3A//www.androidapps.com/tech/articles/9164-hp-abandons-webos-is-apple-too-tough-to-beat

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Choosing The Right Webhosting Company For Your Website | - 0 ...

Choosing the right website hosting company is an important decision. Often, it also the most neglected aspect of the digital economy as companies and individuals are eager to publish their website but not so keen to read the small print of a hosting contract. Before signing up with the web hosting company, make sure that you are completely familiar with their web hosting policy. The most important aspect that you should be familiar with is the traffic charge policy. This is because some web hosting providers charge additional fees for a large amount of traffic that exceeds the given limit while other providers don?t.

A proper web hosting UK and UK web host is important if you?re launching a website on the Internet. Internet has open virtually endless ways for you to make money using a website. You should make sure you get a UK web hosting provider that you can trust, especially if you?re looking to make money off your website. There are thousands of successful and profitable Internet businesses online today. You should do some research about your potential UK webhosting provider, especially if you?re serious about having a successful online business.

Most people think that it all stops at picking the best webhosting provider. This is a common misconception, since you need to constantly monitor your host. This monitoring will be more than just checking to see if your website is up everyday. Your server uptime needs to be monitored constantly. If you?re guaranteed uptime, you can claim compensation from your service provider. You can also know when and for how long your website has been down. The uptime is crucial for your website?s success. Therefore, do check the websites that are being hosted by your chosen web hosting service provider. You want your website to be always easily accessible by your target audience.

A lot of webhosting companies only give their customers a 99% uptime. This is the average, so if you can, try to get a good uptime rate, like 99.5% or higher, so that your website will be continuously online. Although no company guarantees a 100% uptime, some clever researching on your part will land you a company which offers 99.99% uptime. You can lose potential customers everytime your website goes down, which is why it?s important to constantly have your website online.

If you want to manage and promote an online brand, you should consider launching a personal website for it. A personal website would require a unique domain name and a good webhosting service provider. You won?t find this a hard task, since the market is teeming with lots of webhosting companies.

Tags: Web Hosting, webhosting, website hosting

This entry was posted on Wednesday, August 17th, 2011 at 2:04 AM and is filed under Informational Post. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.

Source: http://beforeyoubuyawebsite.com/blog/2011/08/17/choosing-the-right-webhosting-company-for-your-website/

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Tips Of Internet Business Strategies That Are Cost Effective ...

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If you are looking for a cost effective internet marketing strategy, then you have opened the right article. In this article you will find some of the money sure ways so that having an internet business industry is fruitful.

You have to know that cost effective online marketing strategy traditionally combines the benefits of different online tools as well as develops a complete approach to market and promote the targeted service or product. In fact, the internet marketing strategy obviously should include short term and long term plans of actions to reach positive business results.

You have to know that short term online marketing strategies ate helpful in getting temporary boost in the web traffic which could help you convert quite a few visitors into paying customers. As a rule, short term internet marketing strategies include using bulletin boards to post different articles or information concerning the service or product, buying advertising on other websites as well a range of search engine optimization services.

Posting your articles on different bulletin boards and blogs as well as participating in various online forums could improve the visibility of the product or service without spending a dime since these strategies are free to use. But, still this is necessary to check the rules about placing links to website or auto responder in that particular blog or forum because they could have some restrictions about commercial use of that web page.

On the other side, long term internet marketing strategies involve ones that are able to provide you with a constant stream of targeted web traffic over a long period of time. As a rule, these long term strategies guarantee that your visitors and previous customers will go back to your website even after years. These strategies include creating option lists in order to build a database of targeted customers and freebies to boost the popularity and content to ensure the return of the buyer and visitor.

By implementing both long term and short term internet marketing strategies, you are able to gain a reasonable approach towards the online marketing as well as you ensure a steady stream of targeted web traffic to your website. Later this ensures that the online marketing strategy is not just cost effective, but successful as well.

The other tip is online marketing strategy integration. This is a crucial element of the advertising process developed to build web traffic and internet brand awareness. As a rule, it includes different online marketing tolls like permission based email marketing, search engine marketing and partnered marketing which are great cost effective internet based business strategies.

An integrated online marketing service provider could include web designing, creative marketing strategy, managed web hosting as well as web development. All these additional services offer the client company customized internet solutions that satisfies both their long term and short term business needs.

Any site has the right to get internet marketing. Discover how lots of site owners are building targeted visitors today ? this is part of Internet marketing strategy for successful online entrepreneurs shown on this freetrafficsystem.com site.

P.S. And once you have nice targeted traffic ? then ?website traffic? questions become very easy.

P.P.S. Right now we are living in the world where knowledge quickly enhances the quality of our life. Due to this if you are properly armed with the information in your sphere of interest you can be sure that you will always find the way out from any bad situation. So, please make sure to track this blog on a regular basis or ? best of all ? sign up to its RSS feed. In such an easy way you will have a direct shortcut to the latest informational updates here. Blogs can be helpful, you just need to know how to use them.

from your own site.

Source: http://wealth-secret.net/internet-marketing-information/tips-of-internet-business-strategies-that-are-cost-effective/

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A mold on which to create new parts of the puzzle that is the nervous system

Public release date: 16-Aug-2011
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Elhuyar Fundazioa

Biochemist Patricia Garca designs a support in which cells are cultured to treat neurodegenerative diseases, in a thesis defended at the University of the Basque Country

Nervous system diseases (such as Parkinson's or post-traumatic medullar injury) are especially difficult to treat, as it is not easy to replace the parts of the neural puzzle which are damaged. The key is in developing functional neurons from in vitro-treated, cells but for this it is essential that the support on which these cells are based simulate the characteristics of the nervous system. This is what biochemist Patricia Garca has done, developing and validating a polymer support capable of inducing neuronal differentiation in vitro. Her PhD thesis, defended at the University of the Basque Country (UPV/EHU) and undertaken at the Tecnalia Health Unit, is entitled Development of a new polymer support with components of extracellular neural matrix for application in the in vitro differentiation of different primary cells.

Ms Garca has developed a polymer material which contains topographic and biochemical characteristics suitable for converting cells into functional neurones. With this purpose in mind, photolithographic techniques have been combined with the technology of extracellular neural matrixes. The photolithographic technique involves a series of treatments based on exposure to light and chemical substances, and has acted to provide the support with the desired topographical form. As regards the extracellular neural matrix, cells adhere to this natural substrate in order to, amongst other things, be guided and acquire neuronal functionality, enabling the formation of new transplantable tissue for the nervous system. Ms Garca simulated the properties of this matrix in the support developed.

Biocompatible and inductor

At the validation phase, this polymer material proved to be biocompatible with the nervous system. It also acts to induce the formation of neurones sufficiently developed to be able to be used therapeutically, providing the induction is carried out from neural origin cells (the trials with non-neural origin cells gave rise to immature neurones, in the best of cases). Ms Garca undertook validations with the PC12 rat established cell line (used in numerous trials for neuronal differentiation) and with cell cultures of different origins (primary cultures) extracted from rats.

Concretely, in the case of the validations with PC12, the biocompatibility and capacity of neuronal and dopaminergic differentiation (dopamine complies with neurotransmitter functions) of the support developed have been clearly demonstrated.

Only with those of neural origin

As regards primary cultures, Ms Garca carried out trials with cells from the hypocampus in the embryonic rat. These are cells not only of neural origin but with the neuronal line already determined in states of early development such as the embryonic stage. These cells differentiate more efficiently in the developed matrix than in the classic conditions of culture, obtaining mature neurones after only eight days.

The second primary culture validated in the matrix was the one consisting of neural precursor cells (cells at the stage prior to converting into neurones) of the subventricular zone (also the brain) of the neonatal rat. The results show the capacity of dopaminergic neuronal differentiation in less than 30 days of culture. Moreover, an extensive network of glial cells between the matrix and the neurones were observed, and whose function is to give trophic support to and signal the neurones in development.

The last validation trial in primary culture was undertaken with cells of non-neural origin. These are precursor cells derived from the skin of an adult rat, given that they have been already described in the literature as an important source of neural precursor cells. Nevertheless, in this case, the cells stay undifferentiated on the matrix developed, and only in a low percentage have immature neurones been obtained.

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About the author

Patricia Garca Parra (San Sebastin, 1982) is a biochemistry graduate (UPV/EHU) and has three Masters: Molecular Biology and Biomedicine (UPV/EHU), Neuroscience and the Biology of Behaviour (University Pablo Olavide of Seville) and Management of Innovation and Technology (University of Deusto). She drew up her PhD thesis under the direction of Dr. Jos Iaki lava Marqunez (Health Unit, Tecnalia Research & Innovation-TRI) and of Dr. Fabio Cavaliere (Department of Neurosciences of Medicine at the UPV/EHU). She defended her thesis at the Department of Biochemistry and Molecular Biology at the Faculty of Science and Technology. The research was undertaken at the former Inasmet (currently belonging to TRI) with a specialisation grant from the Iaki Goenaga Foundation, and she also collaborated with the Tefilo Hernando Institute at the Universidad Autnoma in Madrid. Currently Ms Garca is a post-doctoral researcher in the Instituto de Investigacin Sanitaria Biodonostia (Hospital Donostia), in this Basque coastal city.

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Public release date: 16-Aug-2011
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Contact: Amaia Portugal
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Elhuyar Fundazioa

Biochemist Patricia Garca designs a support in which cells are cultured to treat neurodegenerative diseases, in a thesis defended at the University of the Basque Country

Nervous system diseases (such as Parkinson's or post-traumatic medullar injury) are especially difficult to treat, as it is not easy to replace the parts of the neural puzzle which are damaged. The key is in developing functional neurons from in vitro-treated, cells but for this it is essential that the support on which these cells are based simulate the characteristics of the nervous system. This is what biochemist Patricia Garca has done, developing and validating a polymer support capable of inducing neuronal differentiation in vitro. Her PhD thesis, defended at the University of the Basque Country (UPV/EHU) and undertaken at the Tecnalia Health Unit, is entitled Development of a new polymer support with components of extracellular neural matrix for application in the in vitro differentiation of different primary cells.

Ms Garca has developed a polymer material which contains topographic and biochemical characteristics suitable for converting cells into functional neurones. With this purpose in mind, photolithographic techniques have been combined with the technology of extracellular neural matrixes. The photolithographic technique involves a series of treatments based on exposure to light and chemical substances, and has acted to provide the support with the desired topographical form. As regards the extracellular neural matrix, cells adhere to this natural substrate in order to, amongst other things, be guided and acquire neuronal functionality, enabling the formation of new transplantable tissue for the nervous system. Ms Garca simulated the properties of this matrix in the support developed.

Biocompatible and inductor

At the validation phase, this polymer material proved to be biocompatible with the nervous system. It also acts to induce the formation of neurones sufficiently developed to be able to be used therapeutically, providing the induction is carried out from neural origin cells (the trials with non-neural origin cells gave rise to immature neurones, in the best of cases). Ms Garca undertook validations with the PC12 rat established cell line (used in numerous trials for neuronal differentiation) and with cell cultures of different origins (primary cultures) extracted from rats.

Concretely, in the case of the validations with PC12, the biocompatibility and capacity of neuronal and dopaminergic differentiation (dopamine complies with neurotransmitter functions) of the support developed have been clearly demonstrated.

Only with those of neural origin

As regards primary cultures, Ms Garca carried out trials with cells from the hypocampus in the embryonic rat. These are cells not only of neural origin but with the neuronal line already determined in states of early development such as the embryonic stage. These cells differentiate more efficiently in the developed matrix than in the classic conditions of culture, obtaining mature neurones after only eight days.

The second primary culture validated in the matrix was the one consisting of neural precursor cells (cells at the stage prior to converting into neurones) of the subventricular zone (also the brain) of the neonatal rat. The results show the capacity of dopaminergic neuronal differentiation in less than 30 days of culture. Moreover, an extensive network of glial cells between the matrix and the neurones were observed, and whose function is to give trophic support to and signal the neurones in development.

The last validation trial in primary culture was undertaken with cells of non-neural origin. These are precursor cells derived from the skin of an adult rat, given that they have been already described in the literature as an important source of neural precursor cells. Nevertheless, in this case, the cells stay undifferentiated on the matrix developed, and only in a low percentage have immature neurones been obtained.

###

About the author

Patricia Garca Parra (San Sebastin, 1982) is a biochemistry graduate (UPV/EHU) and has three Masters: Molecular Biology and Biomedicine (UPV/EHU), Neuroscience and the Biology of Behaviour (University Pablo Olavide of Seville) and Management of Innovation and Technology (University of Deusto). She drew up her PhD thesis under the direction of Dr. Jos Iaki lava Marqunez (Health Unit, Tecnalia Research & Innovation-TRI) and of Dr. Fabio Cavaliere (Department of Neurosciences of Medicine at the UPV/EHU). She defended her thesis at the Department of Biochemistry and Molecular Biology at the Faculty of Science and Technology. The research was undertaken at the former Inasmet (currently belonging to TRI) with a specialisation grant from the Iaki Goenaga Foundation, and she also collaborated with the Tefilo Hernando Institute at the Universidad Autnoma in Madrid. Currently Ms Garca is a post-doctoral researcher in the Instituto de Investigacin Sanitaria Biodonostia (Hospital Donostia), in this Basque coastal city.

---

[ | E-mail | Share ]

?

AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.

Source: http://www.eurekalert.org/pub_releases/2011-08/ef-amo081611.php

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